Improving sensitivity of single tube nested PCR to detect fastidious microorganisms
نویسندگان
چکیده
منابع مشابه
Single-tube nested PCR in the diagnosis of tuberculosis.
AIMS To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment sensitivity and specificity withou...
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Institut f~r Laboratoriumsund Transfusionsmedizin, Herzund Diabeteszentrum Nordrhein-Westfalen, Universit~itsklinik der Ruhr-Universit~it Bochum, Bad Oeynhausen, Germany The application of nested PCR in medical diagnostics implies that the same type of reaction is performed repetitively in the same laboratory over long periods of time, such as in the longitudinal monitoring of immunosuppressed ...
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ژورنال
عنوان ژورنال: Heliyon
سال: 2020
ISSN: 2405-8440
DOI: 10.1016/j.heliyon.2020.e03246